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We have been carrying out the large scale mutagenesis program to develop mutant mice using supermutagen, ENU(N-ethyl-N-nitrourea), and reported the effects of ENU at the dose of 200 and 400 ㎎/㎏. This experiment was designed to decide the optimal dose to get useful model animal pool. 150 ㎎/㎏, and 200 ㎎/㎏, ENU was injected into BALB/cAnNCrj mice at the age of 12 weeks. The lethality, and the sterile period is 12.9%, 11 weeks respectively in the 150 ㎍/㎎ ENU group, and 8.6%, 12 weeks respectively in the 200 ㎎/㎏, ENU group. The average of litter size and the percent of weanlings is 4.7, 87.4% respectively in the 150 ㎎/㎏, ENU group, and 4.2, 84% respectively in the 200 ㎎/㎏, ENU group. In comparison with each group, there are not significant differences, in a sterile period, lethality, litter size, weaning rate. However, because higher dose had more effective mutation rate, we concluded 200 ㎎/㎏, optimal dose.
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